Description
This Mouse Complement Component 1r (C1r) ELISA Kit is intended for quantitative detection of mouse C1R in cell culture supernates, serum and plasma (heparin, EDTA, citrate) and urine. Strip well format. Reagents for up to 96 tests.
This mouse C1R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for C1R has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S18-D705) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for C1R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Complement C1r subcomponent is a protein involved in the complement system of the innate immune system. In humans, C1r is encoded by the C1R gene. It is mapped to 6; 6 F2 and 6 F2; 6. C1r has been shown to interact with C1s. C1r cleaves C1s to form the active form of C1s